O 2 r e q u i r e m e n t = 2 , 604 m g m i n × 1.2 m g O 2 m g B O D = 3 , 125 m g m i n {\displaystyle O2\ requirement=2,604\ {\frac {mg}{min}}\times 1.2\ {\frac {mgO2}{mgBOD}}=3,125\ {\frac {mg}{min}}}